For connective tissue stains – Stain with a poly-acid step
Stain results:
| Nuclei: |
|
Grayish-Black |
| Collagen Fibers: |
|
Red |
| Elastic Fibers: |
|
Bluish-Black |
| Muscle: |
|
Greenish-Yellow |
References:
Gravey, Winsome et al “A modified Verhoff Elastic Van Gieson Stain” The Journal of Histotechnology, Vol. 14, No. 2, pp113 –114. June 1991.