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Flood plate with a suspension of cells in medium. Allow the cells to attach or 1-24 hours in a humidified CO2 incubator.
After cultivation, cells can be washed, fixed and stained in the plate using standard protocols. The hydrophobic silicone creates a fluid barrier to facilitate antibody incubations on coverslips.
Grasp the silicone backing with forceps and pull slowly upward releasing the silicone backing form the plate surface.
Coverslips can be affixed to a slide by means of the tacky silicone backing for routine processing of slides. The silcone backing can also be used to create a water barrier for probe incubations under the coverslip inverted and mounted on a slide (Figure 6). The silicone backing can also be easily removed by peeling it away from the coverslip for routine slide mounting (Figure 7).
SecureSlips™ are for laboratory use only.
Secureslip Cell Culture Coverslips are available online from the EMS Catalog. For ordering or product information, click here.