Herxheimer Technique for Fat
EMS Catalog #: 26500-Series
Fixation:
Formalin.
Sections:
Frozen
Staining Procedures:
- Immerse in 70% Alcohol for an instant.
- Stain for 2 – 5 minutes in Sudan IV Staining Solution (#26500-01).
- Wash quickly in 70% Alcohol. Rinse in distilled water.
- Counterstain in Harris Hematoxylin (#26500-02). Follow with 2 – 5 minutes in Acetic Acid, 1% (#26500-04) for clearer blue staining of nuclei.
- Wash thoroughly in tap water.
- Mount (Glycerin or Glycerin Jelly (#17998-10)
Stain Results:
Nuclei |
Blue |
Fat |
Orange-Red |
Cholesterol |
Red |
References:
Clark, G, ed.: Staining Procedures, 3rd ed.: Baltimore: Williams & Wilkins Co., 1973, p. 143
Conn’s, H.J.: Biological Stains; 8th Ed., Baltimore: Williams and Wilks Co., c. 1969, p. 100
Product Information
Herxheimer Technique for Fat Staining Kit