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10% Buffered Neutral Formalin (#15740) or fixative of choice.
Paraffin at 6µm or Celloidin.
Collagen | Red |
Smooth and striated muscle | Yellowish to brownish |
Cornified epithelium | Yellow |
Hyalin | Yellow |
Nuclei | Blue to Black |
Clark, G., (ed) Staining Procedures, 3rd Ed., William & Wilkins Co., Baltimore, c 1973, p.50
Luna, L. (ed) AFIP Manual of Histological Staining Methods, 3rd Ed., New York, McGraw Hill Publications, c. 1968, p. 76.
Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, c 1968, p.76.
Van Gieson, I., New York Med. J., 50:57-60, 1889.
Mallory, F.B., Pathological Technique, W,B. Saunders Co. Philadelphia 1938.
Mallory, F.B., Pathological Technique, Hafner Publishing., New York, 1961, p 152.